DNA Libraries: Subcloning

This flash animation shows the process of subcloning. Subcloning is part of the process of preparing DNA for sequencing during the Human Genome Project.

This animation shows the major stages of subcloning. This is the process of breaking up fragments of DNA from BAC clones into smaller more manageable pieces for DNA sequencing. This process was very important during the Human Genome Project.

Subcloning involves several standard molecular biology techniques including purifying DNA, gel electrophoresis, restriction digests and ligation. 

This page was last updated on 2015-12-01